Hydrazine derivatized cells

G - Physics – 01 – N

Patent

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Details

G01N 33/554 (2006.01) G01N 33/50 (2006.01) G01N 33/543 (2006.01) G01N 33/556 (2006.01) G01N 33/557 (2006.01) G01N 33/564 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2167931

A method is provided for the preparation of semisolid particles or cells that have been chemically derivatized resulting in hydrazide functionalities covalently incorporated onto cell surface membrane molecules of the semisolid particles or cells, as well as a method for determining the levels of hydrazide groups on the particles or cells. The method involves the preparation and analysis of red blood cells chemically derivatized in such a manner that hydrazide functionalities have been covalently incorporated onto the cell membrane molecules, the preparation of hydrazine derivatized cells and the use thereof of the novel cells in agglutination reactions to promote the attachment of antigens and antibodies to the hydrazine derivatized cells without loss of antibody activity or the blocking of the epitope of interest.

L'invention concerne un procédé de préparation de particules ou de cellules semi-solides qui ont été chimiquement tranformées en dérivés et qui ainsi possèdent des fonctionnalités hydrazide qui sont incorporées de manière covalente sur des molécules de la membranes superficielle cellulaire des particules ou des cellules semi-solides. L'invention concerne également un procédé de détermination des taux de groupes hydrazide se trouvant sur les particules ou les cellules. Le procédé consiste à préparer et à analyser les hématies transformées chimiquement en dérivés de sorte que les fonctionnalités hydrazide soient incorporées de manière covalente sur les molécules de la membrane cellulaire, à préparer les cellules transformées en dérivés de l'hydrazine et à utiliser ces nouvelles cellules dans des réactions d'agglutination afin de faciliter la fixation des antigènes et des anticorps sur les cellules transformées en dérivés de l'hydrazine sans perte de l'activité des anticorps ou sans blocage de l'épitope considéré.

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