Hydridisation assay and reagents

Details Hydridisation assay and reagents Hydridisation assay and reagents

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2180439

An assay for a target nucleotide sequence in a sample, comprises bringing into contact with the sample first and second nucleotide probes respectively labelled with first and second enzymes of a channelling pair, and wherein the probes hybridize at closely adjacent sites on the target sequence in such a way that the two enzyme labels are brought into juxtaposition, and introducing the materials (excluding substrate for the second enzyme that is formed by the first enzymic reaction) which allow the enzymic reactions to run, thereby generating a detectable signal in the presence of the target but not in its absence.

Un dosage destiné à une séquence de nucléotides cible dans un échantillon consiste à mettre en contact avec l'échantillon des première et seconde sondes nucléotidiques marquées respectivement par des première et seconde enzymes d'une paire de canalisation, les sondes s'hydridant au niveau de sites étroitement adjacents sur la séquence cible de manière que les deux marques enzymatiques soient juxtaposées, puis à introduire les matières (à l'exclusion du substrat de la seconde enzyme formée par la première réaction enzymatique) permettant aux réactions enzymatiques d'avoir lieu, générant ainsi un signal détectable en présence de la cible et non en son absence.

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