Hydrogel for an intravenous amperometric biosensor

A - Human Necessities – 61 – B

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A61B 5/00 (2006.01) A61L 27/52 (2006.01) C12Q 1/00 (2006.01)

Patent

CA 2630537

A hydrogel for an intravenous amperometric biosensor is formed on an electrode from a matrix of chitosan and a cross-linking agent such as genipin to immobilize a reactive agent such as glucose oxidase. The matrix may also function as a rate-limiting layer to selectively control diffusion to the reactive agent. A method for forming the hydrogel on an electrode for immobilizing a reactive agent may include dissolving chitosan in an acidic solution, adding the reactive agent, applying solution to the electrode surface, curing the solution to a solid film, and immersing the same in a genipin buffer.

L'hydrogel selon l'invention pour un biocapteur ampérométrique intraveineux est formé sur une électrode à partir d'une matrice de chitosane et d'un agent réticulant tel que la génipine pour immobiliser un agent réactif comme la glucose-oxydase. La matrice peut également servir de couche limitant la vitesse pour contrôler sélectivement la diffusion de l'agent réactif. Le procédé selon l'invention pour fabriquer l'hydrogel sur une électrode pour immobiliser un agent réactif peut consister à dissoudre de la chitosane dans une solution acide, à ajouter l'agent réactif, à appliquer la solution sur la surface de l'électrode, à durcir la solution en une pellicule solide, et à l'immerger dans un tampon de génipine.

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