Hydrogenase polypeptide and methods of use

Details Hydrogenase polypeptide and methods of use Hydrogenase polypeptide and methods of use

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C12N 9/02 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2708108

Provided herein are polypeptides having hydrogenase activity. The polypeptide may be multimeric, and may have hydrogenase activity of at least 0.05 micromoles H2 produced min -1 mg protein -1. Also provided herein are polynucleotides encoding the polypeptides, genetically modified microbes that include polynucleotides encoding one or more subunits of the multimeric polypeptide, and methods for making and using the polypeptides.

L'invention concerne des polypeptides qui présentent une activité hydrogénase. Le polypeptide peut être multimère et peut présenter une activité hydrogénase d'au moins 0,05 micromoles d'H2 produites min-1 mg protéine-1. L'invention concerne également des polynucléotides codant pour les polypeptides, des microbes génétiquement modifiés qui comprennent des polynucléotides codant pour une ou plusieurs sous-unités du polypeptide multimère, et des procédés de fabrication et d'utilisation des polypeptides.

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