C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A01K 67/027 (2006.01) A23C 7/00 (2006.01) A23D 7/00 (2006.01) A23L 1/00 (2006.01) A61K 31/7088 (2006.01) A61K 31/7105 (2006.01) A61K 38/46 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01) C07K 16/40 (2006.01) C07K 19/00 (2006.01) C10M 177/00 (2006.01) C11D 3/386 (2006.01) C12N 9/14 (2006.01) C12N 11/00 (2006.01) C12N 15/10 (2006.01) C12N 15/82 (2006.01) C12P 7/52 (2006.01) C12P 7/62 (2006.01) C12P 7/64 (2006.01) C12P 19/34 (2006.01) C12P 21/08
Patent
CA 2515583
The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phosphlipase A, B, C and D acitvity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzyme, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, the polypeptides of the invention are used to synthesize enantiomerically pure chiral products.
La présente invention concerne des hydrolases, des polynucléotides les codant, et des procédés de fabrication et d'utilisation de ces polynucléotides et polypeptides. Un aspect de l'invention concerne, d'une part des polypeptides, tels que des enzymes, présentant une activité hydrolase (estérase, acylase, lipase, phospholipase activité phospholipase A, B, C et D, activité patatine, activité LAH ("Lipid Acyl Hydrolase") ou une activité protéase, y compris une activité hydrolase thermostable et thermotolérante, et d'autre part des polynucléotides codant cette enzyme. Cet aspect de l'invention concerne également la fabrication et l'utilisation de ces polynucléotides et polypeptides. Les activités hydrolase des polypeptides et peptides de l'invention englobent l'activité estérase, l'activité lipase (hydrolyse de lipides), les réactions d'acidolyse (remplacement d'un acide gras estérifié par un acide gras libre), les réactions de transestérification (échange d'acides gras entre triglycérides), la synthèse d'esters, les réactions d'échanges d'esters, l'activité phospholipase, et l'activité protéase (hydrolyse des liaisons peptides). Les polypeptides de l'invention conviennent à diverses applications pharmaceutiques, agricoles et industrielles, y compris la fabrication de cosmétiques et d'alicaments. Un autre aspect de l'invention porte sur l'utilisation des polypeptides de l'invention pour la synthèse de produits chiraux énantiomériquement purs.
Bornscheuer Uwe T.
Hitchman Tim
Lyon Jonathan
Weiner David Paul
Wongsakul Sirirung
Diversa Corporation
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Verenium Corporation
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