Hydrolysis of protein-bound cholesterol esters

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

150/15, 150/3.3

C12Q 1/00 (2006.01) C12Q 1/25 (2006.01) C12Q 1/60 (2006.01)

Patent

CA 1104041

Abstract of the Disclosure A novel process is described for hydrolyzing protein- bound cholesterol esters such as are found in blood serum. The method comprises contacting sample containing protein-bound cholesterol esters with a compatible mixture of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and, as an effector, a surfactant which is an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units. Hydrolysis compositions comprising compatible mixtures of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and an effector which is a surfactant as described are also disclosed, as are analytical elements com- prising at least one layer which includes such a hydrolysis composition. -1-

294608

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