Hydrolyzable fluorescent substrates and analytical...

Details Hydrolyzable fluorescent substrates and analytical... Hydrolyzable fluorescent substrates and analytical...

C - Chemistry, Metallurgy

12

Q

260/209, 150/9,

C12Q 1/34 (2006.01) C07F 9/12 (2006.01) C07H 15/24 (2006.01) C09B 3/00 (2006.01) C09B 57/00 (2006.01) C12Q 1/44 (2006.01) G01N 33/52 (2006.01)

Patent

CA 1336306

Hydrolyzable substrates comprise blocked dye moieties which, when cleaved from the substrate dur- ing hydolysis, provide fluorescent dyes having maxi- mum absorptions above about 530 nm and maximum emis- sions at least about 580 nm at physiological pH. These substrates can be used in analytical determin- ations of hydrolytic substances including hydrolytic enzymes or biological cells containing such enzymes.

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