C - Chemistry – Metallurgy – 07 – F
Patent
C - Chemistry, Metallurgy
07
F
167/45, 260/461.
C07F 9/12 (2006.01) C12Q 1/42 (2006.01)
Patent
CA 1273023
HYDROLYZABLE FLUORESCENT SUBSTRATES FOR PHOSPHATASES AND ANALYTICAL USE THEREOF Abstract of the Disclosure Hydrolyzable substrates for acid and alka- line phosphatases comprise blocked dye moieties which, when cleaved from the substrate during hydro- lysis, provide fluorescent dyes having maximum ab- sorptions above about 530 nm and maximum fluorescent emissions at least about 580 nm. The dyes are blocked prior to hydrolysis with a phosphono or thi- oxophosphono group or a salt thereof. These sub- strates can be used in analytical determinations of acid or alkaline phosphatase, or in competitive bind- ing reactions to determine immunologically reactive substances.
507346
Babb Bruce E.
Mcclune Gregory J.
Sundberg Michael W.
Babb Bruce E.
Eastman Kodak Company
Gowling Lafleur Henderson Llp
Mcclune Gregory J.
Sundberg Michael W.
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