C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 9/88 (2006.01)
Patent
CA 2400585
The invention provides for a chimeric construct comprising a regulatory region obtained from a hydroperoxide lyase (HPL) gene in operative association with a heterologous gene of interest. Even though the HPL gene is known to be expressed in response to wounding, unexpectedly, it was observed that the HPL regulatory region or one or more fragments thereof, result in constitutive expression of the gene of interest in a range of tissues and organs in the absence of any wound or stress treatment. This invention also provides for a transgenic organism comprising the chimeric construct defined above, and a method for preparing a transgenic organism using the chimeric construct.
L'invention porte sur un gène chimère comportant une région régulatrice obtenue à l'aide d'un gène d'hydroperoxyde lyase (HPL) fonctionnellement associé à un gène hétérologue d'intérêt. Bien que le gène d'HPL soit connu pour être exprimé en réponse à une lésion, on a pu observer de manière inattendue que la région régulatrice, ou un ou plusieurs de ses fragments, résultait de l'expression constitutive du gène d'intérêt dans divers tissus et organes en l'absence de lésion ou de traitement stressant. L'invention porte également sur un organisme transgénique comprenant le gène chimère défini ci-dessus et sur son procédé de préparation utilisant ledit gène chimère.
Bate Nicholas J.
Brown Daniel Charles William
Gleddie Stephen
Hegedus Dwayne
Schafer Ulrike
Gowling Lafleur Henderson Llp
Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food
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