Hydrophobic chromatographic resins with ionizable groups

Details Hydrophobic chromatographic resins with ionizable groups Hydrophobic chromatographic resins with ionizable groups

B - Operations, Transporting

01

J

B01J 20/281 (2006.01) B01D 15/08 (2006.01) B01J 20/286 (2006.01) B01J 20/287 (2006.01) B01J 20/289 (2006.01) B01J 20/32 (2006.01) C07K 1/16 (2006.01) C07K 1/18 (2006.01) C07K 1/20 (2006.01) C12N 9/50 (2006.01)

Patent

CA 2193867

Disclosed are resins, resin-protein/peptide complexes and methods for purifying proteins and peptides using said resins. The resins described herein are useful for the binding of a selected protein or peptide, particularly from an aqueous medium such as a fermentation broth, by hydrophobic interactions between the resin and the selected protein or peptide. The resin is characterized by the fact that it contains ionizable ligands and/or functionalities which are uncharged at the pH of binding the target protein or peptide, thereby facilitating hydrophobic interactions, and charged at the pH of desorption, thereby disrupting the established hydrophobic interaction between the resin and the target protein or peptide. More particularly, the present invention is directed to the use of the described resins in the purification of recombinant enzyme products such as proteases, for example, chymosin or subtilisin.

L'invention concerne des résines, des complexes de résines-protéines/peptides et des procédés de purification de protéines et de peptides à l'aide desdites résines. Les résines de l'invention sont utiles pour la liaison d'une protéine ou d'un peptide sélectionné, notamment provenant d'un milieu aqueux tel qu'un bouillon de fermentation, par interaction hydrophobe entre la résine et la protéine ou le peptide sélectionné. La résine est caractérisée par le fait qu'elle contient des ligands ionisables et/ou présente des fonctionalités non chargées au pH de liaison de la protéine ou du peptide cible, facilitant ainsi les interactions hydrophobes, et chargées au pH de désorbtion, interrompant ainsi l'interaction hydrophobe établie entre la résine et la protéine ou le peptide cible. L'invention concerne plus particulièrement l'utilisation des résines décrites dans la purification de produits enzymatiques recombinés tels que des protéases, par exemple, la chimosine ou la subtilisine.

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