C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/11 (2006.01) C12N 15/70 (2006.01) C12N 15/87 (2006.01) C12N 15/90 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2319168
Mariner-family transposable elements are active in a wide variety of organisms and are becoming increasingly important genetic tools in species lacking sophisticated genetics. The Himar1 element, a member of the mariner family, isolated from the horn fly, Haematobia irritans, is active in Escherichia coli when expressed appropriately. Using this fact, a genetic screen was devised to isolate hyperactive mutants of Himar1 transposase that enhance overall transposition from 4 to 50-fold as measured in an E. coli assay. These hyperactive Himar1 mutant transposases should enable sophisticated analysis of the biochemistry of mariner transposition and should improve efficiency of a variety of genetic manipulations involving transposition in vivo and in vitro such as random mutagenesis or transgenesis in a wide range of host cells than the transposable elements previously available.
Akerley Brian J.
Lampe David J.
Robertson Hugh M.
Rubin Eric J.
Mckay-Carey & Company
President And Fellows Of Harvard College
The Board Of Trustees Of The University Of Illinois
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