Hyperactive mutants of himar1 transposase and methods for...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) C12N 15/70 (2006.01) C12N 15/87 (2006.01) C12N 15/90 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2319168

Mariner-family transposable elements are active in a wide variety of organisms and are becoming increasingly important genetic tools in species lacking sophisticated genetics. The Himar1 element, a member of the mariner family, isolated from the horn fly, Haematobia irritans, is active in Escherichia coli when expressed appropriately. Using this fact, a genetic screen was devised to isolate hyperactive mutants of Himar1 transposase that enhance overall transposition from 4 to 50-fold as measured in an E. coli assay. These hyperactive Himar1 mutant transposases should enable sophisticated analysis of the biochemistry of mariner transposition and should improve efficiency of a variety of genetic manipulations involving transposition in vivo and in vitro such as random mutagenesis or transgenesis in a wide range of host cells than the transposable elements previously available.

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