Hyperactive, non-phosphorylated, mutant transposases of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/55 (2006.01) A61K 38/16 (2006.01) C12N 9/22 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2514730

The invention relates to hyperactive, non-phosphorylated, mutant transposases of mariner mobile genetic elements. The invention also relates to recombinant nucleotide sequences encoding such transposases. The invention further relates to a method of producing said transposases and to the use thereof for in vitro or in vivo transposition.

La présente invention a pour objets des transposases mutantes, non phosphorylables, hyperactives, d'éléments génétiques mobiles mariner. L'invention concerne également des séquences nucléotidiques recombinantes codant de telles transposases. En outre, l'invention vise un procédé de production de ces transposases, ainsi que l'utilisation de ces dernières pour la transposition in vitro ou in vivo.

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