Hyperblebbing bacterial strains and use thereof for...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 1/21 (2006.01) A61K 39/095 (2006.01) A61K 39/10 (2006.01) A61K 39/102 (2006.01) A61K 39/104 (2006.01) A61K 39/106 (2006.01) C07K 14/195 (2006.01) C07K 14/22 (2006.01) C07K 14/285 (2006.01) C12N 1/20 (2006.01)

Patent

CA 2447905

The present invention relates to the field genetically-engineered Gram- negative bacterial strains that have improved outer-membrane vesicle shedding properties, and vaccine compositions comprising these bacteria or vesicles. The present invention provides a hyperbledding Gram-negative bacterium which has been genetically modified by either or both processes selected from a group of consisting of: down-regulation of expression of one or more tol genes; and mutation of one or more gene(s) encoding a protein comprising a peptidoglycan-associated site to attenuate the peptidoglycan-binding activity of the protein(s).

La présente invention concerne le domaine des nouvelles souches de bactéries Gram négatif fabriquées qui présentent des propriétés améliorées d'élimination des vésicules de la membrane extérieure et des compositions de vaccin comprenant ces bactéries ou ces vésicules. Cette invention concerne une bactérie gram négatif hyperboursouflée qui a été génétiquement modifiée par un et/ou deux processus tels que: la régulation restrictive de l'expression d'au moins un gène <i>tol</i>; et la mutation d'au moins un gène codant une protéine comprenant un site associé au peptidoglycane pour atténuer l'activité de liaison avec le peptidoglycane de la (des) protéine(s).

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