Hyperproduction of shoots during in vitro regeneration of plant

A - Human Necessities – 01 – H

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A01H 4/00 (2006.01) A01G 7/00 (2006.01)

Patent

CA 2071767

28 ABSTRACT A process for producing a plurality of viable plant regenerants from a single intact plant seed comprises: i) culturing an intact normally healthy plant seed in a suitable medium, the seed being free of contaminants to the medium; ii) providing in the medium, a growth regulator of sufficient concentration to promote a first growth phase, where at least one shoot is formed from the seed and subsequently inducing a second growth phase in which de nova differentiation in the at least one shoot produces the plurality of regenerants; iii) continuing culture of the seed during the second growth phase until the regenerants are distinct and well formed.

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