Ice seeding apparatus for cryopreservation systems

F - Mech Eng,Light,Heat,Weapons – 25 – D

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F25D 3/10 (2006.01) F25D 17/02 (2006.01) F25D 25/02 (2006.01) F25D 29/00 (2006.01)

Patent

CA 2245004

An apparatus for ice seeding during cryopreservation of biological samples such as cells, harvested tissues, and cellular biological constructs such as culture tissue equivalents wherein ice seeding is performed by discharging a liquefied or chilled gas (60) from a nozzle (21) to the surface of a container (40) containing said biological sample in cryopreservative. Inside a freezing chamber (70), containers (40) are placed on racks (30) that are located proximal to a sprayrail (20) containing nozzles (21). Ice seeding is performed by discharge of liquefied or chilled gas (60) from the nozzles to the container surface at the solid-liquid equilibrium temperature of the cryopreservative. Localized cooling of the container surface by the discharge results in ice seed formation within the container (40).

Dispositif d'amorçage de la congélation pour conservation cryogénique d'échantillons biologiques tels que des cellules, des tissus prélevés et des constructions biologiques cellulaires telles que des équivalents de tissus de culture. Avec ce dispositif, l'amorçage de la congélation s'effectue en libérant un gaz liquifié ou refroidi (60), à partir d'une buse (21), sur la surface d'un contenant (40) abritant ledit échantillon biologique à conserver par le froid. A l'intérieur d'une chambre de congélation (70), les contenants (40) sont placés sur des grilles (30) disposées à proximité d'une rampe de diffusion (20) comportant des buses (21). L'amorçage de la congélation s'effectue par libération d'un gaz liquéfié ou refroidi (60) à partir de ces buses (21) en direction de la surface du contenant, à la température d'équilibre solide/liquide du conservateur cryogénique. Le refroidissement localisé de la surface du contenant, effectué par cette libération, entraîne la formation de glace à l'intérieur du contenant.

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