C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/02 (2006.01) A61K 45/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/04 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2311705
The present invention relates to an improved method for the identification and optionally the characterisation of interacting molecules designed to detect positive clones from the rather large numbers of false positive clones isolated by conventional two-hybrid systems. The method of the invention relies on a novel combination of selection steps used to detect clones that express interacting molecules from false positive clones. The present invention provides for high-throughput interaction screens for the reliable identification of interacting molecules, which in turn can lead to the identification of substances inhibiting said interactions. Such inhibitors can find their use in the formulation of a pharmaceutical composition. The present invention further relates to kits useful for carrying out the method of the invention.
L'invention porte sur un procédé amélioré d'identification et facultativement de caractérisation de molécules interactives conçu pour distinguer les clones positifs d'avec le nombre relativement important de clones faussement positifs isolés à l'aide de systèmes di-hybrides usuels. Ledit procédé repose sur une nouvelle combinaison d'étapes de sélection servant à distinguer les clones exprimant des molécules interactives d'avec les clones faussement positifs. Ladite invention qui permet de filtrer à fort débit les interactions en vue d'une identification fiable des molécules interactives peut en outre conduire à l'identification de substances inhibant lesdites interactions, et pouvant entrer dans la composition de préparations pharmaceutiques. L'invention porte également sur des trousses permettant d'en mettre en oeuvre le procédé.
Bancroft David
Lehrach Hans
Maier Elmar
Meier-Ewert Sebastian
Wanker Erich
Borden Ladner Gervais Llp
Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.v.
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