Identification and cloning of a full-length human...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) A01K 67/027 (2006.01) A61K 38/17 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C12N 5/10 (2006.01) C12Q 1/68 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2423863

The present invention describes a newly discovered full-length polynucleotide encoding an SH2 domain-containing adapter protein, called human MIST, cloned, isolated and identified from a human spleen cDNA library. Also described are the MIST polypeptide sequence, expression vectors, host cells, agonists, antagonists, antisense molecules, and antibodies related to the polynucleotide and/or polypeptide of the present invention. Novel splice variant forms of human MIST are provided. Methods for screening for modulators, particularly inhibitors, of the MIST protein and use of the human MIST polynucleotide and polypeptide for therapeutics and diagnostics are described.

La présente invention concerne un polynucléotide entier découvert récemment qui code une protéine adaptatrice contenant le domaine SH2, appelé MIST humaine, qu'on a clonée, isolée et identifiée à partir d'une banque d'ADNc de rate humaine; la séquence polypeptidique MIST, les vecteurs d'expression, les cellules hôtes, les agonistes, les antagonistes, les molécules antisens et les anticorps relatifs au polynucléotide et/ou au polypeptide selon la présente invention; des formes de variant d'épissage de la MIST humaine; des procédés de criblage de modulateurs, notamment d'inhibiteurs de la protéine MIST et l'utilisation du polynucléotide et du polypeptide MIST humain à des fins de thérapie et de diagnostic.

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