Identification and cloning of a novel human gene, ret16,...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) A61K 31/7088 (2006.01) A61K 38/17 (2006.01) A61P 37/00 (2006.01) C07H 21/00 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C12P 21/02 (2006.01) C12Q 1/02 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2438369

The present invention describes a newly discovered polynucleotide encoding a protein involved in the cell signaling cascade, called RET16, cloned, isolated and identified from TNF-alpha stimulated human microvascular endothelial cells, as well as mouse and rat RET16 orthologs thereof. Also described are the RET16 polypeptide sequence, expression vectors, host cells, agonists, antagonists, antisense molecules, and antibodies related to the polynucleotide and/or polypeptide of the present invention. Methods for screening for modulators, particularly inhibitors, of the human RET16 protein, and use of the RET16 polynucleotide and polypeptide for therapeutics and diagnostics are described.

L'invention concerne un nouveau polynucléotide codant pour une protéine qui joue un rôle dans la cascade de signalisation intracellulaire. Ce nouveau polynucléotide, appelé RET16, est cloné, isolé et identifié parmi les cellules endothéliales microvasculaires humaines stimulées par TNF-alpha, ainsi que leurs orthologues RET16 issus de souris et de rats. L'invention concerne également la séquence polypeptidique RET16, des vecteurs d'expression, des cellules hôtes, des agonistes, des antagonistes, des molécules antisens et des anticorps liés au polynucléotide et/ou polypeptide selon l'invention. L'invention concerne également des méthodes de criblage de modulateurs, notamment d'inhibiteurs, de la protéine humaine RET16, et l'utilisation du polynucléotide et du polypeptide RET16 à des fins thérapeutiques et diagnostiques.

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