Identification and engineering of antibodies with variant fc...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/13 (2006.01) A61K 39/395 (2006.01) C07K 14/705 (2006.01) C07K 16/00 (2006.01) C07K 16/28 (2006.01) C07K 16/30 (2006.01) C12N 15/63 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2512729

The present invention relates to molecules, particularly polypeptides, more particularly immunoglobulins (e.g., antibodies), comprising a variant Fc region, wherein said variant Fc region comprises at least one amino acid modification relative to a wild-type Fc region, which variant Fc region binds Fc.gamma.RIIIA and/or Fc.gamma.RIIA with a greater affinity, relative to a comparable molecule comprising the wild-type Fc region. The molecules of the invention are particularly useful in preventing, treating, or ameliorating one or more symptoms associated with a disease, disorder, or infection. The molecules of the invention are particularly useful for the treatment or prevention of a disease or disorder where an enhanced efficacy of effector cell function (e.g., ADCC) mediated by Fc.gamma.R is desired, e.g., cancer, infectious disease, and in enhancing the therapeutic efficacy of therapeutic antibodies the effect of which is mediated by ADCC.

L'invention concerne des molécules, notamment des polypeptides, et plus particulièrement des immunoglobulines (par exemple des anticorps) comprenant une région du variant Fc, cette région du variant Fc comprenant au moins une modification d'acide aminé par rapport à une zone Fc de type sauvage, la zone du variant Fc se liant à Fc.gamma.RIIIA et/ou Fc.gamma.RIIA avec une plus grande affinité qu'une molécule comparable comprenant la région Fc de type sauvage. Les molécules de l'invention sont très utiles dans la prévention, le traitement ou l'amélioration de un ou plusieurs symptômes associés à une maladie, un trouble ou une infection. Ces molécules sont très utiles dans le traitement ou la prévention d'une maladie ou d'un trouble dans lesquels une efficacité améliorée de la fonction cellulaire effectrice (par exemple ADCC) à médiation de Fc?R est désirée, par exemple le cancer, les maladies infectieuses, et dans l'amélioration de l'efficacité thérapeutique d'anticorps thérapeutiques dont l'effet est à médiation de ADCC.

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