C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.22, 195/1.
C12N 15/00 (2006.01) C07K 14/195 (2006.01) C12N 15/74 (2006.01) C12N 15/78 (2006.01)
Patent
CA 1236036
ABSTRACT OF THE DISCLOSURE Root hair curling is a first step in the infection of soybeans by Rhizobium which leads to nodulation and symbiotic nitrogen fixation. DNA encoding this function has been isolated from two strains R. japonicum and cloned into broad host range vectors. The two 8.7 kb EcoRi fragments found to contain the root-hair curling genes can be transferred to various bacterial genera to confer root-hair curling phenotypes. Pseudomonas putida served as a most suitable host for such expression of root-hair curling gene. Transfer of multicopy plasmid containing these genes enhances the root-hair curling phenotype, and such transfers may enhance competitiveness of the recipients. The location of the root-hair curling gene followed by its expression in a suitable host may allow identification of the product encoded by such genes.
452499
Stanley John
Sutton Benjamin C.s.
Verma Desh P.s.
Allelix Biopharmaceuticals Inc.
Mccarthy Tetrault Llp
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