Identification and isolation of somatic stem cells and uses...

G - Physics – 01 – N

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G01N 33/577 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2457632

The present invention relates to the identification of a specific population of cell types, in particular somatic stem cells including haematopoietic stem cells, mesenchymal stem cells and keratinocyte stem cells. The invention also provides for methods of isolation and uses of the stem cells. Derived from the methods of the present invention, there is provided a method of identifying a stem cell comprising the steps of: obtaining a cell sample including stem cells; detecting the presence of angiotensin converting enzyme (ACE) or a fragment thereof on a cell; and identifying the stem cells having ACE or a fragment thereof.

La présente invention concerne l'identification d'une population spécifique de types cellulaires, en particulier des cellules souches somatiques, et notamment des cellules souches hématopoïétiques, des cellules souches mésenchymateuses et des cellules souches kératinocytes. L'invention concerne également des procédés d'isolement et des utilisations des cellules souches. L'invention concerne en outre, dérivant des procédés de l'invention, un procédé permettant d'identifier une cellule souche. A cet effet, on commence par sélectionner un échantillon cellulaire incluant des cellules souches. On recherche sur une cellule la présence d'un enzyme de conversion de l'angiotensine ou l'un de ses fragments. Il ne reste plus qu'à identifier les cellules souches portant ladite enzyme ou l'un de ses fragments.

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