Identification and quantification of a plurality of...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2652319

Disclosed is a system and method conducting real-time PCR. Unlabeled capture molecules of a specific design are immobilized on a solid support, and contacted with amplicons produced in one or more PCR cycles. Detection of amplicons may take place during or between the PCR cycles while the solid support is in fluidic contact with the PCR solution. In an alternate embodiment detection of the amplicons takes place when the solid support is not in fluidic contact with the PCR solution. The method is suitable for the simultaneous detection and quantification of closely homologous target molecules.

L'invention concerne un système et un procédé permettant d'effectuer une RCP en temps réel. Des molécules de capture non marquées de conception spécifique sont immobilisées sur un support solide et mises en contact avec des amplicons produits lors d'au moins un cycle RCP. La détection d'amplicons peut s'effectuer pendant ou entre les cycles RCP, lorsque le support solide est en contact fluidique avec la solution RCP. Dans un mode de réalisation de variante de l'invention, la détection d'amplicons peut s'effectuer lorsque le support solide n'est pas en contact fluidique avec la solution RCP. Ce procédé est approprié pour détecter et quantifier simultanément des molécules cibles homologues.

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