Identification and use of prognostic and predictive markers...

C - Chemistry – Metallurgy – 12 – Q

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Details

C12Q 1/68 (2006.01) A61K 39/395 (2006.01) A61P 35/00 (2006.01) C40B 30/04 (2006.01) G01N 33/574 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2726817

The present invention provides a method of screening for markers useful in predicting the efficacy of a specified cancer that includes: (a) constructing a tissue microarray from a tissue bank comprising multiple tissue samples that are annotated with clinical follow up data; (b) labeling polynucleic acid probes specific for oncogenes or cancer associated genes known to be potential amplicons; (c) performing fluorescent in situ hybridization analysis on the tissue microarray; and (d) correlating the re-sult of the fluorescent in situ hybridization with the clinical follow up data. The present disclosure also provides methods of treat-ing breast cancer that include screening a breast cancer patient for amplification of one or more of the genes disclosed herein, and treating a patient having amplification of one or more of these genes with a therapeutically effective amount of a compound that interferes with HER2 signaling.

La présente invention concerne un procédé de criblage de marqueurs utiles dans la prédiction de lefficacité dun cancer spécifié qui comprend : (a) la construction dun microréseau de tissu à partir dune banque de tissu comprenant des échantillons de tissus multiples qui sont annotés avec des données de suivi clinique ; (b) le marquage de sondes dacide polynucléique spécifiques doncogènes ou de gènes associés au cancer connus pour être des amplicons potentiels ; (c) la conduite danalyse dhybridation fluorescente in situ sur le microréseau de tissu ; et (d) la corrélation du résultat de lhybridation fluorescente in situ avec les données de suivi clinique. La présente invention concerne en outre des procédés de traitement du cancer du sein qui comprennent le criblage dun patient atteint de cancer du sein pour lamplification dun ou plusieurs des gènes présentement décrits, et le traitement dun patient ayant une amplification dun ou plusieurs de ces gènes avec une quantité thérapeutiquement efficace dun composé qui interfère avec la signalisation HER2.

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