Identification of a human cytomegalovirus gene involved in...

C - Chemistry – Metallurgy – 12 – N

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C12N 7/01 (2006.01) A61K 31/70 (2006.01) A61K 35/76 (2006.01) A61K 39/245 (2006.01) A61K 48/00 (2006.01) C07K 14/045 (2006.01) C12N 7/00 (2006.01) C12N 7/04 (2006.01) C12N 15/01 (2006.01) C12N 15/38 (2006.01) C12N 15/861 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2196207

Infection of human fibroblast cells with human cytomegalovirus (HCMV) causes down-regulation of cell surface expression of MHC class I. A recombinant mutant HCMV which fails to down-regulate class I heavy chain expression is described. A method of controlling down-regulation of MHC class I expression in a cytomegalovirus infected cell, a pharmaceutical composition, a vaccine composition, a method of preventing or reducing susceptibility to acute cytomegalovirus in an individual, and a virus based gene therapy vector are also described.

L'infection de cellules fibroblastiques humaines par le cytomégalovirus humain (CMGVH) provoque la régulation vers le bas de l'expression de la surface cellulaire de la classe I du complexe majeur d'histocompatibilité CMH. On décrit également un CMGVH mutant produit par recombinaison qui est incapable de réguler vers le bas l'expression des chaînes lourdes de classe I, ainsi qu'un procédé permettant de commander la régulation vers le bas de l'expression de la classe I du CMH dans une cellule infectée par le cytomégalovirus, une composition pharmaceutique, une composition de vaccin, un procédé de prévention ou de réduction de la susceptibilité au cytomégalovirus aigu chez un individu et un vecteur de thérapie génique à base de virus.

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