Identification of a human cytomegalovirus gene region...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/38 (2006.01) A61K 39/245 (2006.01) A61K 48/00 (2006.01) C07K 14/045 (2006.01) C12N 7/00 (2006.01) C12N 7/04 (2006.01) C12N 15/861 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2195668

Infection of human fibroblast cells with human cytomegalovirus (HCMV) causes down-regulation of cell surface expression of MHC class I. The present invention is directed to a mutant with a 9-kb deletion in the S component of the HCMV genome (including open reading frames IRS1-US9 and US11) which fails to down-regulate class I heavy chains. By examining the phenotypes of mutants with smaller deletions with this portion of the HCMV genome, a 7-kb region containing at least 9 open reading frames was shown to contain the genes required for reduction in heavy chain expression. Furthermore, it was determined that two subregions (A and B) of the 7-kb region each contained genes which were sufficient to cause heavy chain down- regulation. In subregion B, the US11 gene product is involved. It encodes an endoglycosidase H-sensitive glycoprotein which is intracytoplasmic, similar to the adenovirus type 2 E3-19K glycoprotein which inhibits surface expression of class I heavy chains.

L'infection des cellules du fibroblaste humain par le cytomégalovirus humain (HCMV), rétrorégule l'expression cellulaire superficielle du complexe majeur d'histocompatibilité (MHC) de classe I. La présente invention porte sur un mutant comportant une délétion de 9-kb dans la composante S du génome du HCMV (y compris les cadres de lecture ouverts IRS1-US9 et US11) qui ne rétrorégule pas les chaînes lourdes de classe I. L'examen des phénotypes de mutants à plus faibles délétions de cette portion du génome du HCMV, a montré qu'une région de 7-kb comportant au moins 9 cadres de lecture ouverts contenait les gènes nécessaires pour réduire l'expression des chaînes lourdes. On a de plus déterminé que deux sous-régions (A et B) de la région de 7-kb contenaient chacune des gènes suffisants pour rétroréguler les chaînes lourdes. Le produit génique US11 joue un rôle dans la sous-région B: il code en effet une glycoprotéine intracytoplasmique, sensible à l'endoglycosidase H, et similaire à la glycoprotéine d'adénovirus du type 2 E3-19K qui inhibe l'expression superficielle des chaînes lourdes de classe I.

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