Identification of allele specific nucleic acid sequences by...

Details Identification of allele specific nucleic acid sequences by... Identification of allele specific nucleic acid sequences by...

C - Chemistry, Metallurgy

12

Q

150/8.5

C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 1339979

Hybridization method for discriminating between complementarity and partial complementarity of DNA base sequences. Kinetic covalent entrapment of PCR-amplified target DNA is achieved through the use of crosslinkable probes. A crosslinking site is introduced into the target DNA via the PCR amplification process. Problems with renaturation of target DNA in the PCR process and in the hybridization reaction are minimized.

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