Identification of, and uses for, nematicidal bacillus...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/32 (2006.01) A01H 5/00 (2006.01) A01N 63/02 (2006.01) C07H 21/04 (2006.01) C07K 14/325 (2006.01) C12N 1/20 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2232233

Disclosed and claimed are novel nucleotide primers for the identification of genes encoding toxins active against nematodes and coleopterans. The primers are useful in PCR techniques to produce gene fragments which are characteristic of genes encoding these toxins. The primers are also useful as nucleotide probes to detect the toxin-encoding genes. The subject invention also concerns novel isolates, toxins, and genes useful in the control of plant pests.

Nouvelles amorces nucléotidiques permettant l'identification de gènes codant des toxines actives contre les nématodes et les coléoptères. Ces amorces se révèlent utiles dans les techniques de réaction en chaîne de polymérases, pour obtenir des fragments de gènes caractéristiques des gènes codant ces toxines. Ces amorces se révèlent également utiles comme sondes de nucléotides pour détecter les gènes codant la toxine. L'invention porte enfin sur de nouveaux isolats, de nouvelles toxines et de nouveaux gènes utiles pour la destruction des nuisibles des végétaux.

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