Identification of antigen- or ligand-specific binding proteins

C - Chemistry – Metallurgy – 07 – K

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C07K 16/00 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2715341

The present invention discloses novel methods for the gen-eration, expression and screening of diverse collections of binding proteins such as antibodies or fragments thereof in vertebrate host cells in vitro, for the identification and isolation of ligand- or antigen-specific binding pro-teins. The methods disclosed herein allow the expression of diverse collec-tions of binding proteins from at least one vector construct, which optional-ly can give rise to collections of diverse binding proteins upon transfer and expression into vertebrate host cells in situ.

La présente invention concerne de nouveaux procédés permettant de produire, dexprimer et de cribler divers ensembles de protéines de liaison, tels que des anticorps ou des fragments de ceux-ci dans des cellules hôtes de vertébrés in vitro. Ces procédés permettent didentifier et disoler des protéines de liaison spécifiques à un antigène ou à un ligand. Les procédés présentés ici permettent dexprimer divers ensembles de protéines de liaison provenant dau moins une construction de vecteur, ce qui peut éventuellement donner lieu à des ensembles de protéines de liaison diverses lors de leur transfert et de leur expression dans des cellules hôtes de vertébrés in situ.

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