C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/10 (2006.01)
Patent
CA 2086015
2086015 9119816 PCTABS00008 A modified polymerase chain reaction (PCR) which permits identification of cells characterized by expression of a particular protein, such as B-cells and T-cells is described. The modified PCR selectively amplifies the genes encoding these proteins, which contain both a constant region and a variable region, which variable region contains a consensus sequence. The PCR employs two primers, one of which is a perfect match for the gene encoding a portion of the constant region, and the second primer is a degenerate primer designed to accomodate the gene encoding the consensus sequence. By employing these primers, most of the sequence encoding the protein containing the consensus sequence and constant region can be amplified, cloned and sequenced, thus permitting clonal identification of the cell expressing the protein. The resulting information can be used to design therapies for autoimmune diseases, lymphomas and leukemias.
Danska Jayne S.
Fathman Garrison C.
Ade & Company
Board Of Trustees Of The Leland Stanford Junior University
Danska Jayne S.
Fathman Garrison C.
LandOfFree
Identification of cell subpopulations using modified pcr to... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Identification of cell subpopulations using modified pcr to..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Identification of cell subpopulations using modified pcr to... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1916538