Identification of compound-protein interactions using...

Details Identification of compound-protein interactions using... Identification of compound-protein interactions using...

G - Physics

01

N

G01N 33/543 (2006.01) C40B 30/04 (2006.01) G01N 33/544 (2006.01) G01N 33/551 (2006.01) G01N 33/552 (2006.01) C07K 1/22 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2337490

Disclosed herein is a method for detecting a compound-protein interaction, involving: (a) providing a compound library in which each member of the compound library is immobilized on a solid support; (b) contacting each member of the immobilized compound library in a single reaction chamber with each member of a protein-nucleic acid fusion library under conditions which allow the formation of compound-fusion complexes; (c) isolating the immobilized compound-fusion complexes; and (d) detecting a compound-fusion complex as an indication that the protein of the fusion interacts with the compound. In preferred embodiments, the protein is identified by reading the nucleic acid portion of the fusion, and the compound is identified by reading a detectable tag bound to either the compound or the solid support.

L'invention concerne une méthode de détection d'une interaction composé-protéine consistant: (a) à prendre une banque de composés dans laquelle chaque élément de la banque de composés est immobilisé sur un support solide; (b) à mettre en contact chaque élément de la banque de composés immobilisés, dans une chambre de réaction unique, avec chaque élément d'une banque de fusion protéine-acide nucléique dans des conditions favorisant la formation de complexes composé-produit de fusion; (c) à isoler les complexes composé immobilisés-produit de fusion; et (d) à détecter un complexe composé-produit de fusion indiquant l'interaction de la protéine de la fusion avec le composé. Dans des modes de réalisation préférés, on identifie la protéine en mesurant la partie d'acide nucléique de la fusion et on identifie le composé en mesurant un marqueur détectable lié au composé ou au support solide.

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