G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/68 (2006.01)
Patent
CA 2684948
The invention relates to a method of identifying protein targets that undergo oxidative modification and the specific peptide sequences bearing oxidation-induced modifications. The method further relates to exposing the protein to a condition that results in the modification of an amino acid in the protein, and determining the oxidation state of the protein after exposure to the condition that results in oxidation-induced modification of an amino acid in the protein. The method further compares the oxidation state of the protein before and after exposure to the condition.
La présente invention concerne un procédé d'identification de cibles protéiques qui subissent une modification oxydative et les séquences de peptides spécifiques portant les modifications induites par l'oxydation. Le procédé concerne en outre l'exposition de la protéine à une condition qui entraîne la modification d'un acide aminé dans la protéine, et la détermination de l'état d'oxydation de la protéine après exposition à la condition qui entraîne une modification induite par l'oxydation d'un acide aminé dans la protéine. Le procédé compare en outre l'état d'oxydation de la protéine avant et après exposition à la condition.
Smart & Biggar
The Brigham And Women's Hospital Inc.
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