Identification of protein binding sites

Details Identification of protein binding sites Identification of protein binding sites

C - Chemistry, Metallurgy

40

B

C40B 50/14 (2006.01) C07K 1/04 (2006.01) C07K 17/00 (2006.01) C07K 17/02 (2006.01) C07K 17/06 (2006.01) C40B 30/04 (2006.01) C40B 40/00 (2006.01) C40B 40/10 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2424611

The invention relates to the field of molecular recognition or detection of discontinuous or conformational binding sites or epitopes corresponding to a binding molecule, in particular in relation to protein-protein protein-nucleic acid, nucleic acid-nucleic acid or biomolecule-ligand interactions. The invention provides a synthetic molecular library allowing testing for, identification, characterisation or detection of a discontinuous binding site capable of interacting with a binding molecule, said library having been provided with a plurality of test entities, each test entity comprising at least one first segment spotted next to a second segment, each segment having the capacity of being a potential single part of a discontinuous binding site.

L'invention concerne le domaine de la reconnaissance ou de la détection moléculaire de sites de liaison ou d'épitopes discontinus et conformationnels correspondant à une molécule de liaison, notamment en rapport avec des interactions protéine-protéine protéine-acide nucléique, acide nucléique-acide nucléique ou biomolécule-ligand. Cette invention met en place une librairie de molécules synthétiques permettant de contrôler l'identification, la caractérisation ou la détection d'un site de liaison discontinu capable d'interagir avec une molécule de liaison, cette librairie ayant été alimentée en entités d'essai, chacune de ces entités comprend au moins un premier segment lié à un second segment, chaque segment pouvant être une seule partie d'un site de liaison discontinu.

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