C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2255760
The invention describes a method for the identification of a target bacteria from a sample isolated from a complex matrix such as food and enriched for the presence of the target. The method employs a method of picking colonies cultured from the sample such that the DNA from the cells may be immediately subjected to primer directed amplification after picking and without further processing. Amplification products are detected on the basis of detection of an intercalated fluorescent dye. Fluorescent signal generated from the amplification products is easily differentiated over background.
Méthode d'identification de bactéries cibles dans un échantillon isolé d'une matrice complexe telle qu'un aliment et enrichie pour assurer la présence des bactéries cibles. Cette méthode consiste à repérer les colonies cultivées dans l'échantillon au moyen d'un procédé qui soumet directement l'ADN des cellules repérées à l'amplification initiée par amorce, sans traitement additionnel. Les produits d'amplification sont détectés par une technique de repérage utilisant un colorant fluorescent intercalaire. Les signaux fluorescents générés par les produits amplificateurs sont aisément repérables dans l'échantillon.
Barbour William Mark
Tseng Susan Yen-Tee
Bennett Jones Llp
E.i. Du Pont de Nemours And Company
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