Identification of virus-binding polypeptides and nucleotide...

Details Identification of virus-binding polypeptides and nucleotide... Identification of virus-binding polypeptides and nucleotide...

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01)

Patent

CA 2382646

The present invention provides a novel method for identifying virus-binding polypeptides (putative viral receptors) and the nucleotide sequences encoding them. The method uses a cDNA expression library made from the total mRNA of an animal virus vector and a freshly purified preparation of a plant-infecting virus. Also disclosed are polypeptides identified from the western flower thrips which bind to the tomato spotted wilt virus.

L'invention concerne un nouveau procédé d'identification de polypeptides se liant au virus (récepteurs viraux présumés) et les séquences nucléotidiques les codant. Le procédé repose sur l'utilisation d'une banque d'expression d'ADNc constituée de l'ARNm total d'un vecteur de virus animal et d'une préparation fraîchement purifiée d'un virus infectant les plantes. L'invention concerne également des polypeptides identifiés chez les thrips des fleurs occidentaux qui se lient au virus des taches annulaires de la tomate.

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