Identifying antisense oligonucleotide binding

Details Identifying antisense oligonucleotide binding Identifying antisense oligonucleotide binding

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2267524

A method for identifying an antisense oligonucleotide capable of binding to a target mRNA, which comprises contacting the target mRNA with each member of an oligonucleotide library separately under hybridisation conditions, removing unhybridised material and determining which member or members hybridise; wherein the oligonucleotide library comprises a plurality of distinct nucleotide sequences of a predetermined common length, and wherein each nucleotide sequence comprises a known sequence of 4 to 8 bases and all possible combinations of the known sequence are present in the library.

Procédé d'identification d'un oligonucléotide capable de se lier à un ARNm cible. Le procédé consiste à mettre en contact l'ARNm cible avec chaque élément d'une banque d'oligonucléotides, séparément dans des conditions d'hybridation, à éliminer la manière non hybridée et à déterminer le ou les éléments qui s'hybrident. La banque d'oligonucléotides comprend une pluralité de séquences nucléotidiques distinctes d'une longueur commune prédéterminée, chaque séquence nucléotidique comprenant une séquence connue de 4 à 8 bases, toutes les combinaisons possible de la séquence connue étant présentes dans la banque.

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