Igf-i purification process

Details Igf-i purification process Igf-i purification process

C - Chemistry, Metallurgy

07

K

C07K 14/65 (2006.01) C07K 1/18 (2006.01) C07K 1/20 (2006.01) C07K 1/36 (2006.01)

Patent

CA 2218111

Disclosed is a process for obtaining purified, monomeric, intact, correctly- folded insulin-like growth factor-I (also known as somatomedin-C). The process includes the addition of an IGF-I unfolding/refolding step and the substitution of a reverse phase chromatography step for a gel filtration chromatography step in order to increase the final yield. The process also includes the following steps in order. first cation exchange, unfolding/refolding, hydrophobic interaction chromatography, second cation exchange, and reverse phase chromatography.

L'invention porte sur un procédé permettant d'obtenir un facteur -I de croissance de substances apparentées à l'insuline (également connu sous le nom de somatomédine-C) purifié, monomère, intact, correctement plié. Le procédé comporte une étape supplémentaire de dépliage/repliage d'IGF-I et le remplacement d'une étape de chromatographie par perméation sur gel par une étape de chromatographie à polarité de phases inversée afin d'accroître le rendement final. Le procédé comporte également les étapes suivantes dans cet ordre: premier échange cationique, dépliage/repliage, chromatographie hydrophobe, second échange cationique et chromatographie à polarité de phases inversée.

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