Igm-specific recombinant rheumatoid arthritis antigen

Details Igm-specific recombinant rheumatoid arthritis antigen Igm-specific recombinant rheumatoid arthritis antigen

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/47 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01) G01N 33/564 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2168019

A method is described for diagnosing rheumatoid arthritis by providing a recombinant IgM-specific rheumatoid arthritis-associated antigen and detecting antibodies against the antigen in patient sera. Preliminary steps of making a cDNA library from polyadenylated RNA purified from human cells, selecting recombinant that express the antigen, recloning cDNA containing the antigen gene in a high level expression vector, expressing the antigen in transformed cells, and purifying the antigen are also described.

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