Il-12 gene expression and delivery systems and uses

Details Il-12 gene expression and delivery systems and uses Il-12 gene expression and delivery systems and uses

C - Chemistry, Metallurgy

12

N

C12N 15/24 (2006.01) A61K 31/70 (2006.01) A61K 47/48 (2006.01) A61K 48/00 (2006.01) C07K 14/54 (2006.01) C12N 15/85 (2006.01) C12N 15/88 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2268365

Plasmid expression systems for delivery of DNA coding sequences to a mammal are described which provide expression of multiple coding sequences from a single plasmid. Also desribed are particular lipid/DNA delivery systems having advantageous characteristics of size, charge ratio, and proportion of supercoiled DNA, and methods of preparing and using such delivery systems for treatment.

L'invention concerne des systèmes d'expression plasmidiques destinés à apporter des séquences codantes d'ADN à un mammifère, qui permettent l'expression de multiples séquences codantes à partir d'un seul plasmide. L'invention concerne également des systèmes d'apport de lipides/d'ADN particuliers, ayant des caractéristiques avantageuses en ce qui concerne la taille, le rapport des charges et le pourcentage d'ADN superenroulé, ainsi que des méthodes permettant de préparer et d'utiliser lesdits systèmes d'apport à des fins thérapeutiques.

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