Il-2 gene expression and delivery systems and uses

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/85 (2006.01) A61K 9/127 (2006.01) A61K 48/00 (2006.01) C07K 14/55 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2280190

Plasmid expression systems for delivery of DNA coding sequences to a mammal are described which provide expression of human IL-2. Also described are particular lipid/DNA delivery systems having advantageous characteristics of size, charge ratio, and proportion of supercoiled DNA, and methods of preparing and using such delivery systems for treatment.

L'invention concerne des systèmes d'expression plasmidiques qui permettent d'administrer des séquences codantes d'ADN à un mammifère, de façon que celui-ci exprime l'IL-2 humaine. Elle concerne également des systèmes d'administration de lipides/ADN particuliers qui présentent des caractéristiques avantageuses en ce qui concerne la taille, le rapport des charges et la proportion d'ADN superenroulé, ainsi que des méthodes qui permettent de préparer et d'utiliser lesdits systèmes d'administration pour la thérapeutique.

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