Immobilisation of biological molecules

G - Physics – 01 – N

Patent

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G01N 33/543 (2006.01)

Patent

CA 2612753

A method of immobilising a biological molecule on a substrate comprises: (i) covalently attaching a substantially three-dimensional polysilane polymer to a substrate; and (ii) attaching a biological molecule onto and/or within the polymer. The method may additionally comprise the steps of coating the substrate (or the chemically protective layer) with a layer of radiation sensitive material, exposing the substrate to a source of radiation, and incubating the substrate in the presence of a first species of biological molecule.

L'invention concerne une méthode d'immobilisation d'une molécule biologique sur un substrat consistant (i) à assurer la fixation covalente d'un polymère de polysilane sensiblement tridimensionnel sur un substrat, et (ii) à fixer une molécule biologique sur et/ou dans le polymère. Cette méthode peut en outre consister à revêtir le substrat (ou la couche de protection chimique) d'une couche d'un matériau sensible au rayonnement, à exposer le substrat à une source de rayonnement, et à incuber le substrat en présence d'une première espèce de molécule biologique.

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