Immobilised enzyme electrodes

Details Immobilised enzyme electrodes Immobilised enzyme electrodes

C - Chemistry, Metallurgy

12

M

326/17, 324/28

C12M 1/40 (2006.01) C12N 9/04 (2006.01) C12Q 1/00 (2006.01) C12Q 1/26 (2006.01) C12Q 1/54 (2006.01) G01N 33/66 (2006.01)

Patent

CA 1303132

29 ABSTRACT IMMOBILISED ENZYME ELECTRODES Enzyme electrodes are disclosed which are capable of responding amperometrically to the catalytic activity of the enzyme in the presence of its respective substrate and comprising the enzyme immobilised or adsorbed onto the surface of an electrically conductive support member which consists of or comprises a porous layer of resin-bonded carbon or graphite particles, said particles having intimately mixed therewith, or deposited or adsorbed onto the surface of the individual particles prior to bonding to form said layer, a finely divided platinum group metal, thereby to form a porous, substrate layer onto which said enzyme is adsorbed or immobilised and comprising a substantially heterogeneous layer of resin-bonded carbon or graphite particles, with said platinum group metal dispersed substantially uniformly throughout said layer. The preferred substrate materials are resin bonded platinised carbon paper electrodes comprising platinised carbon powder particles having colloidal platinum adsorbed on the surface of the particles and bonded onto a carbon paper substrate using a synthetic resin, preferably polytetrafluoroethylene, as the binder. The preferred enzyme electrodes are glucose oxidase electrodes comprising glucose oxidase ad- sorbed or immobilised onto the surface of the substrate.

538034

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