C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
324/23
C12N 11/08 (2006.01) C12M 1/40 (2006.01) C12Q 1/00 (2006.01) C12Q 1/26 (2006.01)
Patent
CA 1296060
07-21(542)A IMMOBILISED ENZYMES AND THEIR USE IN AMINOACID ELECTROSENSORS Abstract The enzymes L-methionine gamma lyase, L-lysine decarboxylase, aspartase and tryptophanase are immobilised by contacting the enzyme with a latex of polymer particles having a negative surface charge such that the electrophoretic mobility of the latex has a negative value of -2.0 10 8m2V 1sec 1 or below when measured at pH 7 in 0.01 M KNO3 and a polymer solids concentration of 30 mg/l, the polymer particles also having a hydrophobic surface such that the contact angle of a 1 microlitre droplet of distilled water with a plane horizontal surface formed by drying and compacting the latex particles is 70° or more. The polymer-immobilised enzymes can be used for assaying the corresponding aminoacids in aqueous solutions using an electrosensor comprising a pH- sensitive electrode (6) located in a sensing zone or chamber (1) containing the enzyme/polymer particles as an aqueous dispersion or in water-dispersible form.
581628
Baker Kenneth M.
Baudichau Alain E.
Lardinois Pierre F.
Rouxhet Paul G.
Stone William E.e.
Baker Kenneth M.
Baudichau Alain E.
Lardinois Pierre F.
Mcfadden Fincham
Monsanto Europe S.a.
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