C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
C07H 21/00 (2006.01) C07H 19/10 (2006.01) C07H 19/20 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2365226
A method of immobilising a nucleotide or polynucleotide on a solid support, comprises: providing the solid support having a functionalised surface of the formula B - S - S - L where B is the solid support and L is a leaving group; providing a nucleotide or polynucleotide comprising at least one phosphorothioate group; and coupling the two together by means of a sulphide exchange reaction. The product has the structure B - S - S - P(O)R1R2, where - POR1R2 represents a nucleotide or a polynucleotide. Arrays of the immobilised polynucleotides are useful for performing APEX or SBH assays.
L'invention concerne une méthode servant à immobiliser un nucléotide ou un polynucléotide sur un support solide, consistant à : fournir un support solide présentant une surface fonctionnalisée de formule B - S - S L, dans laquelle B est le support solide et L est un groupe partant ; fournir un nucléotide ou un polynucléotide comprenant au moins un groupe phosphorothioate ; et coupler les deux ensemble grâce à une réaction d'échange sulfurée. Le produit présente la structure B - S - S - P(O)R?1¿R?2¿, dans laquelle - POR?1¿R?2¿ représente un nucléotide ou un polynucléotide. Les ensembles de polynucléotides immobilisés sont utiles pour la mise en oeuvre de techniques APEX ou SBH.
Amersham Biosciences Uk Limited
Amersham Pharmacia Biotech Uk Limited
Fetherstonhaugh & Co.
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