Immobilization of an enzyme substrate

Details Immobilization of an enzyme substrate Immobilization of an enzyme substrate

C - Chemistry, Metallurgy

12

Q

195/138, 252/37

C12Q 1/40 (2006.01) B01D 15/08 (2006.01)

Patent

CA 1109815

ABSTRACT A carbohydrate substrate such as starch for a carbohydrate hydrolyzing enzyme is immobilized on a solid inorganic porous support to form a stable substrate-support composite useful in affinit chromatography and in methods where a precise amount of substrate is needed to perform an enzyme-substrate reaction to quantify the enzyme. The substrate may be activated with an agent such as cyanogen bromide or imidazole prior to deposition on the support so that it may be effectively modified while on the support. After deposition, the substrate is modified by reaction with an epoxyhalogen, aliphatic dihalide or aliphatic diamine to aid in holding it on the support. In an alternative embodiment, the carbohydrate, prior to deposition and modification on the support, is hydrolyzed with an enzyme, preferably dextranase.

323281

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