Immobilization of biocatalyst

Details Immobilization of biocatalyst Immobilization of biocatalyst

C - Chemistry, Metallurgy

12

N

C12N 11/04 (2006.01) C12N 11/08 (2006.01) C12P 13/02 (2006.01) C12P 17/12 (2006.01)

Patent

CA 2540251

Polyacrylamide beads containing encapsulated cells were prepared by a process comprising the steps of (i) providing an aqueous solution of a mixture of acrylic monomers, (ii) providing a suspension of cells in an aqueous solution of a persulfate (iii) providing an emulsion of an aqueous solution of a tertiary amine in a water-immiscible liquid, which liquid optionally contains a surfactant, (iv) mixing the solution provided in step (i) and the suspension provided in step (ii) (v) adding the mixture obtained in step (iv) to the stirred emulsion provided in step (iii) (vi) polymerizing the mixture of acrylic monomers and simultaneously encapsulating the cells to form polyacrylamide beads containing encapsulated cells.

L'invention concerne la préparation de billes de polyacrylamide contenant des cellules encapsulées à l'aide d'un procédé consistant à : (i) préparer une solution aqueuse d'un mélange de monomères acryliques ; (ii) préparer une suspension de cellules dans une solution aqueuse d'un persulfate ; (iii) préparer une émulsion d'une solution aqueuse d'une tertiaire dans un liquide non miscible à l'eau, ce liquide contenant éventuellement un tensioactif ; (iv) mélanger la solution préparée dans l'étape (i) et la suspension préparée dans l'étape (ii) ; (v) ajouter le mélange obtenu dans l'étape (iv) à l'émulsion agitée préparée dans l'étape (iii) ; (vi) polymériser le mélange de monomères acryliques et simultanément encapsuler les cellules afin de former des billes de polyacrylamide contenant des cellules encapsulées.

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