Immobilization of biochemical substances

Details Immobilization of biochemical substances Immobilization of biochemical substances

C - Chemistry, Metallurgy

12

N

C12N 11/08 (2006.01) C07K 17/08 (2006.01) G01N 33/545 (2006.01)

Patent

CA 2092048

ABSTRACT A method for immobilization of biochemical substances, particularly enzymes, which includes the following steps: - An olefin-unsaturated, epoxyfunctional polyether is applied to a carrier material in the form of a layer, - the polyether is cross-linked to form a large-mesh epoxyfunctional polymer matrix by means of high-energy radiation or using pernxide, - the layer is treated with an aqueous solution of the biochemical substance, whereby the biochemical substance is immobilized in the polymer matrix by reaction with epoxy groups, - the layer is stabilized by reaction of non-converted epoxy groups with a compound containing amino and/or carboxyl groups.

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