Immobilization of nucleic acids on solvolyzed nylon supports

Details Immobilization of nucleic acids on solvolyzed nylon supports Immobilization of nucleic acids on solvolyzed nylon supports

C - Chemistry, Metallurgy

07

H

167/44, 195/1.12

C07H 21/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/545 (2006.01)

Patent

CA 1288708

ABSTRACT OF THE DISCLOSURE Immobilization of nucleic acids, e.g., DNA and RNA, by contact with a solid support comprising nylon whose amide groups have been partially solvolyzed. Solvolysis of the nylon support can be accomplished by treatment with an alkylating agent such as a trialkyloxonium salt under anhydrous conditions followed by addition of water. The immobilized nucleic acid is particularly useful as an immobilized probe in hybridization assays to detect specific polynucleotide sequences in a test sample. MS-1419

510924

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