Immobilized alliinase and continuous production of allicin

C - Chemistry – Metallurgy – 12 – N

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C12N 11/00 (2006.01) A23L 1/03 (2006.01) A23L 1/30 (2006.01) A61K 31/21 (2006.01) C12N 9/88 (2006.01) C12P 11/00 (2006.01)

Patent

CA 2251532

Immobilized garlic alliinase wherein the alliinase is chemically, physically or biologically immobilized, is useful in a method for continuous production of allicin. The method comprises adding a solution of alliin as substrate to a column containing said immobilized garlic alliinase and collecting pure allicin in the effluent. The pure allicin is intended for use as food additive or for the preparation of pharmaceutical compositions for the treatment of viral, bacterial, fungal and parasitic infections, high levels of cholesterol and blood lipids, high blood pressure and thrombosis.

L'invention a trait à de l'alliinase de l'ail immobilisée. Cette alliinase, qui a été immobilisée par voie chimique, physique ou biologique, est utilisée dans le cadre d'une technique de production continue d'allicine. Cette technique consiste à introduire une solution d'alliine comme substrat dans une colonne contenant ladite alliinase de l'ail immobilisée, puis à recueillir l'allicine pure dans les effluents. L'allicine pure ainsi produite est destinée à devenir un additif alimentaire ou à entrer dans la préparation de compositions à usage pharmaceutique pour le traitement d'infections d'origine virale et bactérienne ainsi que d'infections dues à des champignons et à des parasites, pour le traitement de teneurs élevées en cholestérol et en lipides du sang ainsi que de thromboses.

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