Immobilized asymmetric double lipid layers

G - Physics – 01 – N

Patent

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Details

G01N 33/50 (2006.01) C07G 99/00 (2009.01) C07K 17/00 (2006.01) C08J 7/04 (2006.01) C12Q 1/00 (2006.01) G01N 33/543 (2006.01) G01N 33/552 (2006.01) G01N 33/553 (2006.01) G01N 33/68 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2448180

The invention relates to a method suitable for the production of immobilized double lipid layers, comprising an inner and an outer lipid layer. The inner and outer lipid layer of said double lipid layers have a defined orientation on the surface of a solid body and are immobilized on the surface of said solid body in a substantially stable and dynamic manner. In order to carry out the inventive method, the surface of a solid body is initially modified in such a way that a surface is formed which preferably interacts with only one of the two lipid layers. In a second step, a double lipid layer is deposited on said modified surface. Preferably, other components, especially protein, are inserted into the double lipid layer.

L'invention concerne un procédé pour réaliser des doubles couches lipidiques immobilisées au moyen d'une couche lipidique intérieure et d'une couche lipidique extérieure. Au niveau de la couche lipidique intérieure et de la couche lipidique extérieure, ces doubles couches lipidiques ont une orientation définie sur la surface d'un solide, sur laquelle elles sont pratiquement immobilisées de manière stable et dynamique. Ce procédé consiste d'abord à modifier la surface d'un solide, de façon à former une surface qui est en interaction, de préférence, avec une seule des deux couches lipidiques. Dans une deuxième étape, une double couche lipidique est déposée sur cette surface modifiée. D'autres composants, notamment des protéines, sont de préférence insérés dans la double couche lipidique.

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