Immobilized cdna libraries

C - Chemistry – Metallurgy – 12 – P

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C12P 19/34 (2006.01) B01J 19/00 (2006.01) C07H 21/00 (2006.01) C07K 17/00 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2343005

Libraries of sense strand cDNA immobilized in the 5'-side. An artificial known base sequence is added to the 3'-side of an antisense cDNA strand (the first strand) and the 5'-side of the second strand is immobilized by using a primer complementary to the above base sequence. Thus, a cDNA library with excellent qualities, which contain the full-length cDNA at a high possibility, can be obtained.

L'invention concerne des banques d'ADNc brin sens immobilisés sur l'extrémité 5', et consiste à ajouter une séquence de base connue, artificielle, à l'extrémité 3' d'un brin d'ADNc antisens (premier brin) et à immobiliser l'extrémité 5' du second brin, en ajoutant une amorce complémentaire de la séquence de base ci-dessus. De cette manière, on obtient une banque d'ADNc possédant d'excellentes qualités et contenant des ADNc complets à fort potentiel.

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